Study design
Primary cell culture
Virus production, infection, and selection
Antibodies
Western blot analysis
Immunoprecipitation assay
Phosphorylation assay for Flt1
Nrp1 antibody treatment
Histology and immunostaining
Image acquisition
FACS isolation of epithelial cells for RNA sequencing
RNA extraction and real-time RT-PCR
List of primers
ATAC-seq and library preparation
ATAC-seq analysis
Motif analysis
GO analysis
Quantification and statistical analysis
K14-Cre (34) mice were mated with Rosa26-VEGF-164 (35), Nrp1fl/fl (36), and Flt1fl/fl (37) mice. All mice used in this study were composed of males and females with mixed genetic background. Mouse colonies were maintained in a certified animal facility in accordance with the European guidelines and with approved ethical protocol (no. 526N).