Medium was removed, and cells were washed once with 1× Dulbecco’s phosphate-buffered saline (Thermo Fisher Scientific). Genomic DNA extraction was performed by addition of 100 μl freshly prepared lysis buffer [10 mM tris-HCl (pH 7.0), 0.05% SDS, and proteinase K (25 μg/ml; Sigma-Aldrich)] directly into the 48-well culture well. The extraction solution was incubated at 37°C for 60 min and then 80°C for 20 min.

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