Western blotting was performed using standard protocols. We used primary antibodies against α-SMA, β-actin, and GAPDH. We used goat antibodies to mouse and rabbit conjugated with horseradish peroxidase as secondary antibodies (Jackson ImmunoResearch) and developed the blots using enhanced chemiluminescence (Dura, Pierce).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.