The study included three male patients and three age- and gender-matched controls. Samples from patients with HF were obtained from Beijing Anzhen Hospital of the Capital University of Medical Sciences at the time of cardiac transplantation. Normal hearts (nonfailing hearts as controls) were obtained from donors with normal cardiac contractile function based on echocardiography of those who had died from motor vehicle accidents. The three male donors had a median age of 48 years. The three HF patients (male) had a median age of 46 years, and their average EF% was 20 ± 5%. The study was performed in accordance with the Declaration of Helsinki and approved by the Institutional Review Board of Beijing Anzhen Hospital, Capital Medical University. Patients provided written consent. Heart tissues were fixed in neutral buffered formalin solution, embedded in paraffin, and then cut into 5-μm serial sections. Immunohistochemistry staining was performed as described previously (44). Monoclonal antibody UCHL1 (1:100 dilution) and BNP (1:100 dilution) were used as primary antibodies.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.