All experiments were approved by the Animal Care and Use Committee of Dalian Medical University and performed in accordance with the U.S. National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals (publication no. 85-23, 1996). C57BL/6J WT and Uchl1gad-2J (UCHL1+/−) mice were purchased from the Jackson laboratory (Bar Harbor, ME, USA). All mice were previously backcrossed over 10 generations to the C57BL/6 background. For all in vivo studies, age- and sex-matched WT and UCHL1+/− mice were used with at least five mice per genotype.

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