Acoustic recordings and analyses
This protocol is extracted from research article:
Inconsistent sexual signaling degrades optimal mating decisions in animals
Sci Adv, May 15, 2020; DOI: 10.1126/sciadv.aax3957

Analyses of male calling behavior were based on recordings made of calling males in our study population. A total of 20 advertisement calls were recorded for each of 50 actively calling males (1000 calls total) at night (2200 to 0100 hours) during active breeding choruses between May and July of 2006 and 2009. Recordings were made using a Sennheiser ME66 microphone with a K6 power supply (Sennheiser USA, Old Lyme, CT, USA) and a Marantz PMD670 Recorder (D&M Professional, Itasca, IL, USA). The microphone was held by hand or positioned on a tripod such that the recording tip of the microphone was approximately 1 m from the calling male. All recordings were made in the dark, and care was taken to allow the recorded male to adjust to any disturbance presented by the arrival of the human observer. Recordings only commenced after subjects had resumed normal calling behavior following any disturbance.

The durations of calling bouts and the instantaneous call rate (equivalent to the inverse of the call period) for each recorded advertisement call were determined using Raven v1.3 (Cornell Lab of Ornithology, Ithaca, NY, USA). Call rates were temperature corrected to 20°C following the methods of Platz and Forester (36). Coefficients of variation within males were computed as each individual male’s SD in call rate scaled by his own mean call rate across the 20 calls (CVw = SD/X¯). We used the curve estimation function in SPSS version 21 (IBM Corporation, Armonk, NY, USA) to examine the relationship between call rate and inconsistency.

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