The I-TASSER protein structure and function prediction server (https://zhanglab.ccmb.med.umich.edu/I-TASSER/) (60) was used to generate a homology model of HAdV-D8 based on the published sequence of HAdV-D8 (22), using the published structure of its closest relative (by sequence identity), HAdV-D19p (37). The resultant monomer was then copied three times, using the HAdV-19p trimer as a template and the monomers aligned in PyMOL to generate a model of the complete HAdV-D8K trimer.

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