All siRNAs were purchased from Dharmacon as pools of four individual siRNAs (ON-TARGETplus SMARTpool). Transfections of pDCs with siRNA were performed using DOTAP (Sigma-Aldrich), as previously described (17). Transfections of siRNA into HEK293T cells were performed using HiPerFect Transfection Reagent (Qiagen), according to the manufacturer’s instructions. Transfection of MoDCs with siRNA was performed using INTERFERin (Polyplus-transfection), according to the manufacturer’s recommendations.

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