Cells were lysed in IP lysis buffer containing 50 mM tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, EDTA-free protease inhibitor cocktail (Roche), and 1 mM phenylmethylsulfonyl fluoride. Cell lysates were incubated overnight at 4°C with anti-Flag M2, anti-HA, or anti–c-Myc agarose affinity gel antibody (Sigma-Aldrich) or anti-IRF7 or anti-Pin1 antibodies and protein G Sepharose beads (Thermo Fisher Scientific). Beads were washed three times and eluted with 2× SDS loading buffer. For immunoblotting, proteins were resolved by SDS–polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane.

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