MNFs were purified as described by the Krishma Halai laboratory. Briefly, skin was isolated from newborns at day 1, incubated in Krebs-Ringer Hepes (KRH) buffer containing 10 mM Hepes and 1% penicillin/streptomycin and minced in pieces with a scalpel before digestion in KRH/ATB buffer supplemented with collagenase (1 mg/ml) at 37°C. After centrifugation at 1000 rpm for 5 min, the supernatant was discarded, and the isolated cells put in culture in DMEM, 20% FBS, and 1% penicillin/streptomycin.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.