293T, A549, HeLa, HeLa-S3, WT-MEF, MEFSting−/−, MEFcGas−/−, MEFTrex1−/−, T98G, and T98GF/H-cGAS were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 1% glutamine. T98G overexpressing F/HA-cGAS were generated by transducing parental T98G with viral particles produced by using the pOZ-F/HAcGAS construct and selected with puromycin. Cells were maintained in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin, and 1% glutamine. A549 were obtained from S. Goodborn. WT-MEF, MEFSting−/−, and MEFcGas−/− were a gift of S. R. Paludan, MEFTrex1−/− were obtained from J. Rehwinkel, and parental T98G were obtained from C. Goujon.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.