The present study aimed at identifying the molecular mechanisms governing regulation of STING activation. Experiments performed in vitro, in cells, and in vivo were carefully controlled. In vitro experiments performed using recombinant proteins or cell lysates were performed independently using different protein preparations. Experiments performed in cells were performed in technical duplicates or triplicates, and biological repetitions are indicated in the figure legends. In vivo experiments in zebrafish were repeated twice on pools of three individual larvae. Morpholino injections were blinded. No outlier was excluded in the course of the analyses.

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