Commercial antibodies to the indicated murine antigens were used: CD11c-phycoerythrin (PE)/Cy7, CD11c-allophycocyanin (APC) (N418), CD11c-APCCy7 (N418), CD64-PE (X54-5/7.1), F4/80-PerCP/Cy5.5, F4/80-APC (BM8), I-Ak-PE (10-3.6), Ly-6C-PacBlue (HK1.4), Ly-6C-BV510 (HK1.4), CD45-PacBlue (30-F11), CD45-BV785 (30-F11), and Ly-6G-PacBlue (1A8) (all from BioLegend San Diego, CA); CD11b-fluorescein isothiocyanate (M1/70), CD11b-PeCy7 (M1/70), and CD11c-PE (N418) (all from eBioscience, San Diego, CA); CD45-BV510 (104), SiglecF-PE, SiglecF–Alexa Fluor 647, SiglecF-APC700, and (E50-2440) Ly6G-BUV395 (1A8) (all from BD Biosciences, San Jose, CA); and MerTK-PE/Cy7 (DS5MMER) (from Invitrogen, Carlsbad, CA). BrdU labeling was performed using the BrdU-APC labeling Kit (BD Biosciences) according to the manufacturer’s protocol. Cells were acquired either on the BD Biosciences LSRFortessa or with a BD FACScan flow cytometer with DxP multicolor upgrades by Cytek Development Inc. (Woodland Park, NJ) and then analyzed using FlowJo software (FlowJo LLC, Ashland, OR). Samples were preincubated with Fc-block [Hybridoma 2.4G2, American Type Culture Collection (ATCC)].

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