Five hundred microliters of processed plasma (centrifuged at 10,000g for 10 min) was overlaid on size exclusion columns filled with Sepharose 4B resins (Sigma Aldrich, Australia) and followed by elution with PBS. The fractions were then collected to determine the particle and protein concentrations via the Micro BCA assay. High particle/low protein fractions were pooled and concentrated in an Amicon Ultra-2 50-kDa centrifugal filter device for the ELISA assay.

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