Antibodies (B5 or B5×1A10) (10 mg/kg) were administered to naïve mice intravenously, and then serum was collected at 0.05, 3, 8, 24, 48, 96, 168, 240, 336, 432, 528, and 624 hours after administration. The serum concentration of antibodies was measured by ELISA against hB7-H3 protein with HRP-conjugated goat anti-human IgG F(ab′)2.

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