Tumor tissue and tdLN were harvested and weighed at 4 days after the last treatment in the MC38hB7-H3 palpable tumor model. Tumor tissues were chopped and digested with 400 Mandl units of collagenase D (Roche, 11088882001) and deoxyribonuclease I (200 μg/ml; Roche, 11284932001) for 30 min at 37°C. Digested tumor tissues were dissociated into a single-cell suspension using a 100-μm cell strainer. tdLN was dissociated into a single-cell suspension using a 40-μm cell strainer. All single-cell suspensions are then maintained in the cIMDM for analysis.

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