Purified 1D8, B5, and B5×1D8 were prepared in NuPAGE LDS sample buffer (Invitrogen, NP0007). For reducing samples, 2-mercaptoethanol (Sigma-Aldrich, M3148) was added in the sample buffer and heated at 70°C for 10 min. A total of 2.5 μg of each antibody and Novex Sharp Pre-stained Protein Standard (Invitrogen, LC5800) was loaded into the wells of NuPAGE 4-12% Bis-Tris Mini Protein Gel (Invitrogen, NP0321). After electrophoresis, the gel was stained by Imperial Protein Stain solution (Thermo Fisher Scientific, 24615).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.