Purified 1D8, B5, and B5×1D8 were prepared in NuPAGE LDS sample buffer (Invitrogen, NP0007). For reducing samples, 2-mercaptoethanol (Sigma-Aldrich, M3148) was added in the sample buffer and heated at 70°C for 10 min. A total of 2.5 μg of each antibody and Novex Sharp Pre-stained Protein Standard (Invitrogen, LC5800) was loaded into the wells of NuPAGE 4-12% Bis-Tris Mini Protein Gel (Invitrogen, NP0321). After electrophoresis, the gel was stained by Imperial Protein Stain solution (Thermo Fisher Scientific, 24615).

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