We use 6-week-old female C57BL/6 (Orient Bio Inc.), 6- to 8-week-old female in-house bred BALB/c, and 6- to 12-week-old 4-1BB KO mice (C57BL/6 background; a gift from B. Kwon, University of Ulsan, Korea). All mouse strains were maintained under specific pathogen–free (SPF) conditions in an approved animal facility at POSTECH Biotech Center. All animal experiments were conducted according to the protocols approved by the Institutional Animal Care and Use Committee of POSTECH.

h4-1BB KI mice [B-hCD137 (4-1BB) mice, C57BL/6-Tnfrsf9tm1(TNFRSF9)/Bcgen/Bcgen, stock number: 110004], whose 4-1BB gene is replaced by the h4-1BB gene, were supplied by Jiangsu Biocytogen Co. Ltd. These mice were housed in an SPF barrier at the animal center of Beijing Biocytogen Co. Ltd. Eight-week-old female h4-1BB KI mice were acclimated for 3 days to 1 week after arrival and used for experiments in Biocytogen Haimen Animal Center.

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