Five microliters of protein solution containing liquid-like droplets or nanofibers was dropped on a piece of clean glass slide (1.0 ± 0.2 mm in thickness; Citoglas) and then covered by a piece of cover glass (0.13 to 0.16 mm; microscopic cover glass, Citoglas). DIC imaging was performed on a ZEISS Z2 microscope equipped with Axiocam 506 camera (ZEISS). The 3D confocal images of microfluidic channel or microtubule were obtained with a Leica TCS SP8 confocal microscope. The 561-nm laser was used for excitation of QDs. Data are presented in Figs. 2F and 4 (C and D).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.