Typically, for sample preparation for AFM imaging, 200 μl of protein solution containing assembled structures was incubated on a mica surface for 30 min to allow sample deposition on mica surface. The excessive solution was then washed away twice with 200 ml of deionized water. The mica surface was dried through a constant flow of nitrogen gas and then immediately collected for AFM imaging. Tapping mode AFM was performed on an Asylum MFP-3D AFM (Asylum Research) using microcantilevers (k = 26.1 N/m and V = ~300 kHz). Data are presented in Fig. 2B and fig. S2A.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.