To visualize the protein coatings formed on substrates and along the inside wall of channel, a solution containing red QDs was carefully injected into the channel and incubated for 1 hour and thoroughly washed with deionized water to remove the loosely bound QDs. Photographs were taken under UV light (254 nm). The adsorption of QDs onto protein coatings was based on metal coordination chemistry between His-tags and Ni-NTA–decorated QDs. Coating images were captured using camera (Canon EOS-3), and 3D images were recorded using a confocal microscope. Data are presented in Figs. 2A and 4 (A, C, and D) and fig. S8B.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.