To monitor the formation of liquid-like droplets in protein solutions containing different concentrations of monomers, turbidity of absorbance at 600 nm was applied as an indicator. TLC-M absorbance was negligible at 600 nm. The relative turbidity is defined as ln(T/T0), where T and T0 are the light transmittance with and without sample, respectively (2). Protein solutions were added into cuvette and measured on Spectramax M5 (Molecular Devices) at different time intervals. Data are presented in Fig. 2D and figs. S3A and S4C.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.