The superficial skin infection model has previously been described (15). Briefly, mice were infected with a preoptimized dose of SN1 or NS33. Mice were sacrificed at the noted time points, and the skin biopsy sample, spleen, and blood were collected. Tissues were homogenized, serially diluted, and plated on blood agar plate for enumeration of bacteria. Blood samples were also used for detection of toxin using Western blots assays. To model acute onset of STSS, the HLA-B6 and B6 mice were also challenged intraperitoneally as previously described (14). Briefly, mice were administered S. pyogenes (at varying doses) intraperitoneally in a 400-μl volume. To assess bacterial burden, mice were culled at defined time points; spleen and blood samples were harvested, serially diluted, and plated. For immunotherapy experiments following skin infection, mice were intraperitoneally administered 200 μl of designated antisera. For immunotherapy following intraperitoneal infection, mice intravenously received 200 μl of various antisera.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.