The J8, J14, and J14.1 peptides were synthesized and conjugated to DT as described (16). rSpeC was purchased from Toxin Technology (USA) and has been previously described (44). To produce the rM1 protein, the gene encoding M1 protein (amino acids 13 to 455) was cloned into pGEX-2T (GE Healthcare Life Sciences), incorporating a C-terminal 6× His-tag as previously described (45).

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