All animal procedures were performed in compliance with the guidelines for the welfare of experimental animals issued by the Federal Government of Germany. F1 heterozygous mice were produced by breeding F0 DBAxC57BL/6J founders to C57BL/6J mice.

Pups were weaned at 19 to 23 days after birth, and females were kept separately from males. The mice were housed in standard individually ventilated cages. General health checks were performed regularly to ensure that any findings were not the result of deteriorating physical conditions of the animals.

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