U2OS nuclei were stained for 20 min using 1:100 Alexa Fluor 647–conjugated anti-H3K27me3 antibody in phosphate-buffered saline 0.1% bovine serum albumin and then washed twice with the same buffer before fluorescence-activated cell sorting (FACS) analysis. Unstained cells were used as negative control. Cells were analyzed by FACS as previously described (30).

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