293T and HCT-116 cells were treated with 10 μM 5-AZA (Sigma-Aldrich) dissolved in DMSO. Treatment was administered only once for each time course experiment performed in this study. HCT-116 was also treated for 4 days with daily administration of 10 μM 5-aza-2′-deoxycytidine. DMSO was used as vehicle control. U2OS cells were treated with 5 μM EZH2 inhibitor EPZ6438 (Selleckchem) dissolved in DMSO. EPZ6438 treatment was administered every other day for the entire duration of the experiment (6 days pretreatment +5 days growth curve). DMSO was used as vehicle control. Growth curves were performed as previously described (30).

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