Because the concentration of NaBH4 was much higher than that of 4-NP, it can be considered to have remained constant throughout the reaction. Accordingly, the reaction was considered as corresponding to a pseudo–first-order reaction model with respect to the concentration of 4-NP, and the reaction constant (k) was calculated from Eq. 3ln(CtC0)=kt(3)where Ct is the concentration of 4-NP at the specified time t, C0 is the initial concentration, and k is the first-order rate constant (s−1).

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