ChIP assay was performed as follows (50). Cells were fixed with 0.8% formaldehyde for 10 min, and then formaldehyde was quenched with 125 mM glycine. The cells were lysed for 20 min on ice, and DNA was fragmented by adding Micrococcal Nuclease (MNase, Thermo Fisher Scientific, 88216) for 20 min at 37°C. The chromatin fragments were then added to Protein A/G beads (Invitrogen, 10004D/10002D) that were attached to H3K4me2 antibody (Millipore, 07-030). Samples were washed twice with radioimmunoprecipitation assay (RIPA) buffer, twice with RIPA high-salt buffer, twice with LiCl wash buffer, and twice with 10 mM tris-HCl (pH 8). DNA was eluted and extracted using AMPure XP beads (A63881, Beckman Coulter Genomics). ChIP libraries were prepared according to the Illumina Genomic DNA protocol.

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