In-gel digestion of gel-separated proteins was carried out with trypsin, as previously described (38). After digestion and extraction from the gel pieces, the digested peptides were desalted and concentrated by reversed-phase chromatography onto C18 Stage Tip microcolumns (39). Peptides were then eluted from the stage tips with buffer B (80% acetonitrile and 0.5% acetic acid), lyophilized, resuspended in 0.1% formic acid (FA), and subjected to liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.