For the validation of specific regions, ChIP-qPCRs were performed as follows. Immunoprecipitated DNA was diluted in 9.6 μl of H2O per reaction, plus 400 nM primers in a final volume of 20 μl in SYBR Green. Each ChIP experiment was performed at least three times with biological replicates. For ChIP-qPCRs, the following primers were used: negative control forward, AGCTATCTGTCGAGCAGCCAAG; negative control reverse, CATTCCCCTCTGTTAGTGGAAGG; PRAM1 forward, CCACAGAGCCTCCCCTAGA; PRAM1 reverse, TGCAACACCTCCCTGTGA; peptidase inhibitor 16 (PI16) forward, AGCCCTCACAGATGAGGAGA; PI16 reverse: GCCACACTTACCATGTGCAG; Integrin subunit alpha (ITGAM) forward, GGAGGAGAAGTGACATGGCT; ITGAM reverse, AGGCAAAGTGGAGATGGTGA; transgutaminase 2 (TGM2) forward, CAGATACAGACACACGCAGC; TGM2 reverse, TGGGGAGGTGTTCTTGATCC; CD86 forward, ACAGTCATTGCCGAGGAAGG; CD86 reverse, CTCATCCGTGTGTCTGTGCT; GFI1b forward, CAGGGAGGGGAACAGAAGAG; and GFI1b reverse, GAACTGCAAAGCCTCTCTCG.

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