NB4 cells, treated as indicated for 24 hours, were transferred by cytospin to histological glasses and fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 5% normal goat serum. Staining was performed using a mouse anti-human PML antibody (PGM3) to reveal PML nuclear bodies or micro-speckles (34). A Cy3-conjugated goat anti-mouse (Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA) was used as secondary antibody.

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