For standard SILAC labeling, NB4 cells were grown in “light” and “heavy” SILAC RPMI 1640 (Thermo Fisher Scientific, 89984) supplemented with either l-arginine and l-lysine or their heavy isotope counterparts l-arginine-13C6, 15N4 hydrochloride (Arg10; Sigma-Aldrich, 608033) and l-lysine-13C6, 15N2 hydrochloride (Lys8; Sigma-Aldrich, 608041) (32). All media were supplemented with 10% dialyzed fetal bovine serum (FBS) (26400-044, Gibco, Life Technologies), 1% glutamine, penicillin (100 U/ml), and streptomycin (100 mg/ml).

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