Fluorescence intensity levels were measured by Fiji (https://imagej.net/Fiji/Downloads). Regions of interest of 30 μm long for D. melanogaster, or the complete length for in vitro polymerized MTs, accounting for the full width of the motoneurons/MTs were used. The levels of acetylated α-tubulin and total α-tubulin levels were extracted from mean intensity levels. Background levels were subtracted, and the ratio of acetylated α-tubulin/tubulin was calculated.

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