Larval locomotion assays were performed by placing third-instar D. melanogaster larvae in the center of 15-cm petri dishes coated with 3% agar (42). Millimeter paper was glued below each 15-cm petri dish to quantify distance traveled. Crawling speed was extrapolated from distance traveled in 1 min; peristaltic activity was defined as complete posteroanterior contraction of the larvae in 1 min. Climbing assay for adult flies was performed as previously described in (43). Ten flies per group were allowed to climb an empty polystyrene tube for 1 min in three sequential assays. Climbing index was defined as average ratio successful climbs over 15 cm of the total number of flies in a group.

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