BDNF-mCherry was previously used in (24) and ATAT1-GFP (27099), pCALNL-DsRed (13769), and pEGFP α-tubulin K40Q (105302) were purchased from Addgene ( pEGFP-Tub was obtained from Clontech ( pCAG mEmerald-LAMP1 and pCAG mito-DsRED were provided by F. Polleux (Columbia University, New York, USA), and pCAG-iCreERT2 was provided by A. Tye (NIMR, UK). pCX-Cre plasmid was designed and provided by X. Morin (Institut de Biologie de l’Ecole Normale Superieure IBENS, France). ATAT1 truncation constructs, FLAG-ATAT1(amino acids 1 to 333), FLAG-ATAT1(amino acids 1 to 286), FLAG-ATAT1(amino acids 1 to 242), and FLAG-ATAT1(amino acids 1 to 196) were synthesized as gBlocks and inserted to a pCIG2 vector. The Atat1 short hairpin RNA (shRNA) sequence was 5′-GCAGCAAATCATGACTATTGT-3′ (30). Atat1 sequence was inserted in pBS/U6-ploxPneo plasmid (provided by X. Coumoul). Lis1 shRNA sequence was 5′-GAGATGAACTAAATCGAGCTA-3′ (38) and was subcloned in pCA-b-EGFPm5 silencer 3, a gift from M. Vermeren (King’s College London, UK). All construct sequences were verified by Sanger sequencing.

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