Frozen human hepatocyte samples were purchased from Lonza Walkersville Inc. Whole livers for hepatocyte isolation were obtained with the informed consent of families of registered organ donors. The obtained liver organs were rejected for transplant due to either lack of a donor match or morphological alterations (e.g., tearing and hematoma). All 12 selected hepatocyte donors were healthy participants of various age, gender, and ethnicity (table S1) without any liver cancer or other liver pathology history. These cells had been isolated using a gold standard, two-step liver/liver lobe perfusion procedure. Cells were suspended in 2 to 5 ml of media and counted with Trypan blue to estimate viability (higher than 80%), and frozen in dimethyl sulfoxide/liquid nitrogen ( One specimen of frozen human neonatal LSCs from a 1-year-old donor was purchased from Kerafast Inc. ( These cells had been derived by the Sherley laboratory (Boston, MA, USA) and characterized to confirm their stem cell identity (3941).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.