Mouse primary neuron immunofluorescence

Cells were washed with 1× PBS three times, fixed in 4% paraformaldehyde (PFA) for 15 min, and blocked in 1× PBS buffer with 5% bovine serum albumin and 0.1% Triton X-100 (PBST) for 30 min at room temperature. The cells were incubated with primary antibodies overnight at 4°C, washed three times in 1× PBS, and then incubated with secondary antibodies at room temperature for 60 min. The signals were observed via fluorescence microscopy.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.