Mouse primary neuron immunofluorescence

Cells were washed with 1× PBS three times, fixed in 4% paraformaldehyde (PFA) for 15 min, and blocked in 1× PBS buffer with 5% bovine serum albumin and 0.1% Triton X-100 (PBST) for 30 min at room temperature. The cells were incubated with primary antibodies overnight at 4°C, washed three times in 1× PBS, and then incubated with secondary antibodies at room temperature for 60 min. The signals were observed via fluorescence microscopy.

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