Calculation of fluorescent plate reader LOD and quantitation

LOD and LOQ were calculated from the construction of an eight-point calibration curve with concentration ranging from 0.10 to 10 or 15 nM for QSH or PS. LOD and LOQ were calculated on the basis of the SD of the response signal of the blank and slope of the linear curve through zero (equations below)LOD=3σS(8)LOQ=10σS(9)where σ is the SD of the blank (NM suspension in trypsin) and S is the slope of the calibration curve. All readings were performed on a Tecan M200 Pro microplate reader.

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