A paralleled series of cell kinetic samples containing QSH were analyzed for uptake using confocal microscopy. At each time point in the study, cells were washed twice, trypsinized, and transferred to 35-mm petri dishes containing 2 ml of complete growth medium. After 24 hours, cells were washed twice with complete growth medium, and Hoechst 33257 was applied. Cells were then imaged for NM uptake with twenty 2-μm step sizes.

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