Anti-CD4 (GK1.5) and/or anti-CD8 β (Lyt3.2 clone 53-5.8; Bio X Cell) monoclonal antibodies were administered at doses of 0.1 mg per mouse to pVAX-tpaNS1 DNA–vaccinated mice by the intraperitoneal route on day −2 before ZIKV challenge. To confirm that CD4 + T cell depletion was maintained through the acute phase of infection, splenocytes were collected and analyzed on days 2 and 5 after treatment. Antibody depletions were >99.9% efficient as determined by flow cytometry using the following antibodies: α-CD4-APC-Cy7 (clone RM4-5), α-CD8-PerCP-Cy 5.5 (clone 53-6.7), and α-CD3-AF700 (clone 500A2).

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