Bone marrow–derived hMSCs were derived from the posterior iliac crest of two healthy male donors (26 and 41 years of age) using a protocol approved by the University Hospitals of Cleveland Institutional Review Board. Cells were isolated using a Percoll gradient (Sigma-Aldrich, St. Louis, MO) and cultured in low-glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, St. Louis, MO) containing 10% fetal bovine serum (Sigma-Aldrich), 1% penicillin/streptomycin (P/S; Thermo Fisher Scientific), and fibroblast growth factor–2 (10 ng/ml; R&D Systems, Minneapolis, MN) (39, 41, 48, 58).

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