PC9 cells were cultured in 96-well plates at 2000 per well confluence overnight. Cells were treated with serial dilution of mAbs together with MMAE-conjugated secondary goat anti-mouse IgG antibody (2 μg/ml) for 72 hours. Cell number was then calculated by Cell Counting Kit-8 (CCK8; Dojindo, no. CK04-20). Antibody-drug conjugation services were provided by Levena Biopharma, Nanjing.

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