Proteomic antigens including membrane, nuclear, or whole-cell lysates were labeled with biotin using EZ-Link NHS-LC-Biotin reagent and incubated with the PETAL arrays. Following a similar procedure as described above, antibody spots positive in three independent experiments were then ranked by the averaged fluorescence intensities. A limited number of array-positive antibodies (1000 to 2000 according to the expected output of each screening) with high (>10,000), medium (2000 to 10,000), and low (500 to 2000) fluorescent intensity were selected as candidate antibodies for further validation assays.

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