The diversity of the PET peptide library (15,199 peptides; see table S1 for detail) is evaluated by comparing the sequence similarity of all peptides against each other. The sequence identity (%) between a peptide and its closest homologs within the library was recorded. PET sequence similarity to two random peptide libraries generated computationally was also compared. The first library was a collection of 15,199 peptide sequences randomly sampled from all species without considering amino acid preference in different species (45). The second library was constructed by randomly sampling the entire human proteome (all consensus coding sequences).

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