Mice were individually placed in clean, ethanol-sterilized cages, and immediately upon defecation, each fecal pellet was placed in a sterile collection tube, snap frozen, and stored at −80°C. Whole blood was collected from anesthetized animals by cardiac puncture. Serum was isolated by centrifugation at 1500g × 15 min and stored at −80°C. Liver, spleen, and intestine were collected immediately after euthanasia. Each tissue was weighed, and colon length was measured before being placed in microcentrifuge tubes, snap frozen, and then stored at −80°C. Hematocrit was determined using a microhematocrit capillary tube reader. Whole colonic tissue was homogenized in cell lysis buffer, and protein concentration was determined using the Precision Red Protein Assay (Cytoskeleton). Samples were sent to Eve Technologies for analysis using their 32-multiplex Mouse Cytokine/Chemokine Array.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.