All animal procedures were approved by the University of Calgary Animal Care Committee (protocol AC14-0068) and were in accordance with the Canadian Council on Animal Care policies. Female C57BL/6 mice aged 7 to 10 weeks (Charles River) were used in all experiments. Mice were housed in sterilized, filter-top cages under specific pathogen–free conditions, separated by treatment group, and fed control chow (Teklad Global 16% protein rodent diet TD.00217) for a minimum of 1 week before commencing any experiment. MMF-containing chow was administered ad libitum at a concentration of 0.563% (w/w) (30). Vancomycin (500 mg/liter) was administered in the drinking water ad libitum. Consumption of both chow and water was monitored daily.

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