Cells were treated with the indicated concentrations of compounds for 24 h at 37°C in the incubator and were subsequently harvested and fixed with 75% ethanol overnight at 4°C. Ribonuclease I (50 μg/ml) was incubated with the cells in PBS at 37°C for 30 min, and then PI (50 μg/ml) was used to stain the cells for 15 min. Flow cytometry and FACSDiva software, v 6.2 (BD Biosciences), were used to perform cell cycle assays. The distribution of the cell cycle phases was analyzed by FlowJo software.

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