Antiproliferative assays of the compounds against different cancer cell lines were performed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays. Cells were seeded into 96-well plates at 3000 to 6000 cells per well and incubated for 24 hours. Subsequently, the cells were treated with the indicated concentration of compounds or the same amount of DMSO for 72 hours. MTT (5 mg/ml) was added and incubated at 37°C for 4 hours. Then, the solution was removed, and 150 μl of DMSO was added to dissolve MTT formazan crystals. Last, the OD values at 570 nm were determined by an ELx800 absorbance microplate reader (BioTek, Vermont, USA). The IC50 values were calculated by nonlinear fit curves using GraphPad Prism 6.0 software.

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