HCT116, A549, H460, L02, HepG2, 786-0, HT1080, MCF-7, SKBR3, PC3, and HT29 cell lines were obtained from the Cell Resource Center of Shanghai Institute for Biological Sciences, Chinese Academy of Sciences. PANC-1 and SW480 were provided by J.Z. (Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Ministry of Education, Shanghai Jiao Tong University School of Medicine). The MCF-7, SKBR3, and PC3 cell lines were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS). HCT116, A549, H460, L02, HepG2, 786-0, HT1080, HT29, NCM460, and CCD18C0 cell lines were maintained in RPMI 1640 medium supplemented with 10% FBS. All cells were authenticated by short tandem repeat profiling, examined for mycoplasma contamination, and cultured at 37°C in a humidified, 5% CO2-containing atmosphere incubator (Thermo Fisher Scientific).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.